I got my results from Enterolab today

wonderwoman · Post by **wonderwoman** » Mon Mar 22, 2010 4:30 pm

The test results are as follows.

Final Laboratory Report Date: 3/22/2010

A) Gluten Sensitivity Stool and Gene Panel Complete *Best test/best value

Fecal Anti-gliadin IgA:    32 Units (Normal is less than 10)

Fecal Anti-tissue Transglutaminase IgA:    11 Units (Normal is less than 10)

Quantitative Microscopic Fecal Fat Score:    Less than 300 Units (Normal is less than 300)

Fecal Anti-casein (cow's milk) IgA:    7 Units (Normal is less than 10)

HLA-DQB1 Molecular analysis, Allele 1:    0202

HLA-DQB1 Molecular analysis, Allele 2:    0603

Serologic equivalent: HLA-DQ   2,1  (Subtype 2,6)

Soy Sensitivity Stool Test
Fecal Anti-soy IgA:    14 Units (Normal is less than 10)

From the above tests I understand

I indeed do have a gluten sensitivity and the enzyme to break down the gluten is ineffective making my body susceptible to developing autoimmune disorders. I knew I was gluten sensitive but happy to see the confirmation from the lab. (I was diagnosed with hypothyroidism in 1999, an autoimmune disorder)

Digestion and absorption of nutrients is currently normal. Wonderful news!

Casein does not present an immune reaction at the present time, however, I will continue to eliminate dairy products.

My DNA 0202 is not a Celiac gene

My DNA 0603 indicates that I have a predisposition of developing neurological problems due to gluten sensitivity

The soy sensitivity test indicated I am sensitive to soy and will continue to eliminate soy in my diet. I am glad I called the lab and had them add this test since I was using soy milk for several years only because of the convenience. I switched to almond milk about a month ago.

I am so happy that I had the tests done. Had I not, I would always have wondered, especially with casein and soy.

I did not understand the meaning of the DNA test results and the above is what they explained to me. I hope I got it right. If not, please tell me. I asked what she meant by neurological disorders and she named about 10. I said I didn't have any of them that I knew of. Her reply was that is possible but what they found is that many people with the 0603 DNA develop neurological disorders. She did mention ADD as one of them and sometimes I think I have some ADD although I would not admit it anywhere but here.

Gabes-Apg · Post by **Gabes-Apg** » Mon Mar 22, 2010 4:41 pm

Charlotte
hope you are well this week, congrats on getting the results, i know you have been very keen to get them.

I am sure some of the guru's will explain meanings etc.
good luck with the ingredient adjustment.. there is lots of soy hidden in so many foods.

starfire · Post by **starfire** » Mon Mar 22, 2010 4:53 pm

Looks like you are the same as me for food sensitivities. I'm gluten and soy also. I thought gluten was everywhere but soy is worse. I was really happy that dairy was normal.

Love, Shirley

Post by **tex** » Mon Mar 22, 2010 8:13 pm

Charlotte,

That's very interesting about the HLA-DQB1 0603 gene. I wasn't aware that they have discovered connections between that gene and neurological issues. I note that Joan, Rick, (ccranch), and Connie, (Stanz), also have copies of that gene. Quite a few of us do have neurological issues, even though we don't have a copy of that gene, myself included.

Yes, I believe that you have interpreted the test results correctly. You are one of relatively few members here who have tested negative to casein intolerance. Lucky you.

Do you mind if I add your test results to our tabulated lists?

Thanks,
Tex

wonderwoman · Post by **wonderwoman** » Mon Mar 22, 2010 11:07 pm

Tex, you are certainly welcome to add my test results to your tabulated list.

MaggieRedwings · Post by **MaggieRedwings** » Tue Mar 23, 2010 5:43 am

Morning Charlotte,

Great that you got back the results and they definitely make one feel better just to know that your assumptions and suspicions are valid. Great on not having cassein a factor but boy you are right - soy is everywhere.

Love, Maggie

Polly · Post by **Polly** » Tue Mar 23, 2010 6:48 am

Good Morning, Charlotte!

How nice to finally know! You are so lucky compared to me and all of my intolerances. I am very interested in the connection between the 603 gene and neurological problems. Was that info also from Dr. Fine's lab? BTW, we have tons of ADD in my family, too!

To your continued success in putting MC in remission,

Polly

harma · Post by **harma** » Tue Mar 23, 2010 10:36 am

This is what I have read on the Enterolab website, about the DQ 1 and 3 genes and the relationship with MC and gluten ataxia. I've copied the text from the website (the whole story can be found under FAQ result interpretations).

"My and other published research has shown that DQ1 and DQ3 also predispose to gluten sensitivity, and certain gluten-related diseases (microscopic colitis for DQ1,3 in my research and gluten ataxia for DQ1 by another researcher). And according to my more recent research, when DQ1,1 or DQ3,3 are present together, the reactions are even stronger than having one of these genes alone (like DQ2,2, DQ2,8, or DQ8,8 can portend a more severe form of celiac disease). "

Post by **tex** » Tue Mar 23, 2010 12:14 pm

Polly,

I found a few research articles, regarding the HLA DQB1*0603 gene. The first one listed is in reference to MS patients:

High-resolution HLA genotyping analysis revealed a robust relationship between alleles HLA-DRB1*1501, -DQB1*0301, -DQB1*0302, -DQB1*0602, and -DQB1*0603, and more severe damage on inflammatory and neurodegenerative MRI measures.

http://www.ncbi.nlm.nih.gov/pubmed/17531857

Here's an article that reports a clear association of HLA DQB1*0603 with hepatitis B virus-associated membranous nephropathy:

HLA DQB1*0603 was increased in patients with HBVMN compared to controls (chi2 = 13.65, RR = 4.3).

http://www.nature.com/ki/journal/v61/n4 ... 2903a.html

Here's an abstract linking HLA DQB1*0603 with juvenile chronic arthritis:

We suggest that those ANA positive individuals with a restricted HLA background, DQB1*0603 positive), defines a group of EOPA-JCA patients which will be especially valuable in the characterization of the ANA associated with EOPA-JCA.

http://rheumatology.oxfordjournals.org/ ... 461?ck=nck

Here's an abstract about Type 1 Diabetes:

http://www3.interscience.wiley.com/jour ... 1&SRETRY=0

This abstract links it with familial sarcoidosis, (I'm not sure whether or not sarcoidosis is officially classified as an autoimmune disease, but it is obviously immune system connected):

http://www.sciencedirect.com/science?_o ... 46c4cfabb8

However, this particular heterozygous combination, (DQB1*603/604), has been found to be protective of MS, according to this article:

http://revista.inmunologia.org/Upload/A ... /2/325.pdf

I found a lot of article leads that I couldn't follow, because many special medical publications require payment, in order to view an article.

Love,
Tex

wonderwoman · Post by **wonderwoman** » Tue Mar 23, 2010 12:52 pm

In answer to Polly's question and I quote Polly here

I am very interested in the connection between the 603 gene and neurological problems. Was that info also from Dr. Fine's lab?

Yes, I spoke to the nurse at EnteroLab and that is what she told me. I asked if there was something more she could send me regarding this and she said no, because they don't do the DNA testing there, it is sent out and they only report it. I HOPE I GOT THAT LAST PART RIGHT.

In regard to what harma and Tex said, I don't understand what they are talking about. Is it important for me to understand more about my DNA????

My question is, with my DNA, will my children be prone to celiac or not?????

Post by **tex** » Tue Mar 23, 2010 2:46 pm

Charlotte,

To the best of my knowledge, the only things that you need to be concerned about, regarding your genes, are that you do have genes that predispose you to gluten sensitivity, and one of them predisposes you to neurological issues. Generally speaking, though, as long as you avoid all sources of gluten, in your diet, no neurological symptoms should develop.

Each of your children inherited one of your genes, (for non-celiac gluten sensitivity), plus a single equivalent gene from your husband, (of unknown sensitivity status). Statistically, half of your children inherited a copy of the HLA-DQB1 0202 gen, and the other half inherited a copy of your HLA-DQB1 0603 gene. Unless your husband possesses a celiac gene, (DQ2 or DQ8), none of your children should have a celiac gene, (at least, they didn't receive one from you). IOW, your children might develop gluten sensitivity, but theoretically, at least, they should not be susceptible to classic celiac sprue, (unless any of them inherited a celiac gene from your husband). The odds of your husband having a celiac gene, are about 43%, (IOW, 31% of the general population have the DQ2 gene, and 12% have DQ8 gene). Of course, if your husband happens to have two copies of a celiac gene, then every one of your children would have inherited a celiac gene from him.

You are correct that Enterolab sends out the gene tests to another lab - actually, they send it to a Red Cross laboratory. Most people are not aware of this, but Enterolab only orders/reports half the full test results, (which also cuts the cost in half - that's why they can offer these tests at about a third of the amount charged by the competition). Actually, a full celiac gene heterodimer, consists of two adjacent gene alleles. For example, for the DQ2 celiac gene, it would be represented as DQA1*0501 and DQB1*0201, which encodes the two subunits, DQ alpha5 and DQ beta2. Note that the Enterolab test results omit the DQA part, (the alpha component). IOW, they only report the beta component, (the DQB part), of the result. For all practical purposes, (as far as we are concerned, this distinction is irrelevant, because while it is true that by omitting the alpha component, their reporting will not meet the criteria for classic celiac disease, instead, it simply refers to a predisposition to gluten sensitivity. Since the treatment is the same, the distinction is irrelevant. Here's what Enterolab says about this, in order to justify their position, (and I totally agree with their decision to do this - just be aware of why they are reporting these test results in this manner).

In the genetic assessment for the predisposition to celiac disease (a disease defined as villous atrophy and inflammation of the small intestine resulting from gluten sensitivity - the immune response to dietary gluten), the heterodimer HLA-DQA1*0501 and HLA-DQB1*0201 is one of the main genes required to develop the tissue lesion of celiac disease. However, it appears from research studies that HLA-DQ1, HLA-DQ2, and HLA-DQ3 can all lead to immune reactions to gluten (i.e., gluten sensitivity) even in the absence of intestinal villous atrophy. Non-celiac gluten sensitivity syndromes are no less severe than ones accompanied by villous atrophy, in terms of symptoms they may cause, accompanying autoimmune diseases in other parts of the body, and resultant disability. Thus, it is more important to assess for genetic predisposition to the underlying immune reactivity to gluten itself than just the one clinical form called celiac disease. Furthermore, fecal analysis cannot diagnose celiac disease, therefore EnteroLab makes no such claims of doing so.

In weighing the potential clinical benefit to our clients of adding HLA-DQA1 locus typing (to our current practice of typing only the HLA-DQB1 locus) versus the almost doubling of cost and price, we have found through internal research that the frequency wherein HLA-DQ2 positivity is the celiac-related DQB1*0201 allele verses the non celiac 0202, 0203, 0204, or 0205 alleles is overwhelming in favor of the celiac 0201 (and we now even separate these subtypes). By linkage dysequilibrium, HLA-DQA1*0501 will usually accompany HLA-DQB1*0201. Furthermore, the presence of HLA-DQ2 assessed serologically (functional presence of any DQ2 gene product) has always shown itself to be able to bind gliadin, and therefore predispose to gluten sensitivity (even if someone did not develop celiac disease/villous atrophy). For these three reasons (the overwhelming prevalence of the 0201 allele of DQ2 in our clientele, the linkage dysequilibrium of DQA1*0501 with DQB1*0201, and the greater importance of diagnosing the underlying immune reactivity to gluten, regardless of syndrome caused), we have decided to keep the price down for our clients and limit gene testing to the HLA-DQB1 locus. Finally, the treatment for gluten sensitivity and celiac disease is the same - complete-strict removal of gluten from the diet.

Tex

wonderwoman · Post by **wonderwoman** » Tue Mar 23, 2010 5:56 pm

I went back and read some prior posts when I came across this one written by TEX

There is also almond dream, which is an almond milk. If I recall correctly, Diamond brand almond milk contains soy

I have soy intolerance and when I read this I went in the refrigerator to see what brand I am using. Sure enough, it is Diamond brand and yes it does contain soy lecithin. I have 4 half gallons in the refrigerator as I had $1.00 off coupons plus they were on sale for $2.69 each. That purchase didn't prove to be a bargain after all. I don't know why I didn't notice that when I bought the first one.

Post by **tex** » Tue Mar 23, 2010 7:43 pm

That's easy to do, and most of us have done it before.

Tex

Polly · Post by **Polly** » Wed Mar 24, 2010 6:48 am

Thanks for the additional info, Charlotte and Harma.

And Tex, thanks for those references. Will check them out.

Love,

Polly

wonderwoman · Post by **wonderwoman** » Wed Mar 24, 2010 12:17 pm

I found this web site for gluten and other food sensitive people and subscribed to their newsletter which contains coupons and recipes. I really am not into doing a lot of fancy recipes or baking, I prefer the foods just plain. But did find a recipe for a GF Granola that I will try and also a crock pot Oatmeal in todays newsletter. I haven't had oatmeal for over a month now and thought I might try it again for a change from GM Rice Chex.

www.befreeforme.com

I have been eating quite a few mixed nuts now which I never did before. I find I can't eat just one or eight for that matter.

Also with strawberries in season now I have been eating a lot of them. Today I read in a Home Remedies book to avoid strawberries because they contain a lot of sorbitol, which our bodies cannot easily digest. I have not noticed any difference since eating them. I can see sorbitol as an additive but when it is in food naturally. Any thoughts on this.